2MD, Ophthalmology, Giresun University Faculty of Medicine, Giresun, Turkey
3Associate Professor, MD, Pathology, Gazi University Faculty of Medicine, Ankara, Turkey
4Associate Professor, MD, Ophthalmology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Turkey DOI : 10.37845/ret.vit.2022.31.66 Purpose: To evaluate the safety of a new intraocular mitomycin c (MMC) application technique in the rabbits.
Materials and Methods: Sixteen male New Zealand rabbits were used. The rabbits were divided into 4 study groups. 2?g MMC in group-A, 5?g MMC in group-B, 10?g MMC in group-C and 20 ?g MMC in group-D were applied with MMC sandwich technique into right eyes of rabbits. In MMC sandwich technique, ciliary body was protected with air, and posterior pole was protected with perfluorocarbon. The left eye was used as a control group. The rabbits had one-month followed up. Intraocular pressures were measured at first week and month 1. Scotopic and photopic a- and b-wave amplitudes and implicit times were compared between control and study eyes. Multiple small slices that included the ciliary body, macula and optic nerve head were examined for the evaluation of MMC toxicity.
Results: Intraocular pressure did not differ between the control and study eyes. Scotopic and photopic a- and b-wave amplitudes and implicit times of group A, B, C and D eyes were similar to those of the control eyes. In all of the study groups, the histopatholologic morphologies of the ciliary body, retina and optic nerve were similar to those of control group. Furthermore, retinal layer thicknesses were similar between the MMC injected eyes and the control eyes.
Conclusions: Our newly defined MMC sandwich technique permitted the use of MMC up to 20 ?g concentrations without causing toxicity in the vital intraocular structures.
Keywords : Intraocular drug application, Mitomycin C, Toxicity, Retinal detachment, Proliferative vitreoretinopathy