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¹Niksar Devlet Hastanesi, Niksar- Tokat
²Dokuz Eylül Üniversitesi Tıp Fak Göz Hastalıkları AD, İzmir
³Dokuz Eylül Üniversitesi Tıp Fak Klinik Mikrobiyoloji ve Mikrobiyoloji AD, İzmir
⁴Dokuz Eylül Üniversitesi Tıp Fak Multidisiplin Laboratuvarı, İzmir Purpose: The growth behaviours of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans commonly associated with endophthalmitis in vivo with silicone oil were investigated.
Methods: 8 rabbits which were New Zelland type, white, male, weighing 2-3 kg were included into the study. Suspansions were prepared from the 24 hours cultures of microorganisms. 0.1 ml suspansion including 10⁷ / ml microorganisms were injected into the eye of rabbits. 48 hours after the injection two different surgical approach were performed for each microorganism. In 4 eyes pars plana vitrectomy was performed and in the remaining 4 eyes silicone oil was injected after pars plana vitrectomy. Sample materials were taken from the operated eye at the 10th day and inoculated into the cultures, colony counting was done as well.
Results: Endophthalmitis was observed clinically in all eyes 24 hours after injection. Staphylococcus aureus did not grow in either vitrectomised eyes or vitrectomised and silicone oil injected eyes at the 10th day. Colony- forming units of Staphylococcus epidermidis decreased in silicone oil injected eyes as Pseudomonas aeruginosa. Candida albicans did not grow in vitrectomised eyes and did grow in silicone oil injected eyes.
Conclusion: It was found that silicone oil had a characteristic that negatively effects the growth of microbiological agents due to its physical and chemical properties and so inhibiting the growth of microbiological agents in silicon enriched media. Keywords : Endophthalmitis, silicone oil, pars plana vitrectomy